Composite

Part:BBa_K2334013:Design

Designed by: Hongbin Yu   Group: iGEM17_SCU-WestChina   (2017-10-20)


J23113 4.1.1.97 + J23106 pucM + K2334001


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 782
    Illegal NheI site found at 805
    Illegal NheI site found at 1170
    Illegal NheI site found at 1401
    Illegal NheI site found at 1424
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 203
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1054


Design Notes

They're used for urate metabolic pathway construction.


Source

Bacillus Subtilis & Klebsiella pneumoniae

References